Label-free bioelectronic detection of aptamer-protein interactions

ABDEL-N. KAWDE; MARCELA C. RODRIGUEZ; THOMAS M. H. LEE; JOSEPH WANG

ELECTROCHEMISTRY COMMUNICATIONS

Elsevier

Lugar: Amsterdam; Año: 2005 vol. 7 p. 537 - 537

1388-2481

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: justify; mso-layout-grid-align: none">We demonstrate for the first time the utility of nucleic acid aptamers for electrochemical detection of proteins. Highly specific and sensitive label-free detection of the target protein is achieved by combining aptamer-coated magnetic beads and chronopotentiometric stripping measurements of the captured protein (in connection to the intrinsic electroactivity of the protein). Lysozyme has thus been detected selectively in a mixture containing a large excess of six proteins and amino acids (both electroactive and non-electroactive), with a detection limit of 350 fmol (7 nM). While aptamer-based electronic sensors are in their infancy, such devices offer attractive opportunities for electrochemical detection of pro